Herpetofaunal Communities in Ephemeral Wetlands
Embedded within Longleaf Pine Flatwoods of the Gulf
Coastal Plain
Kenneth J. Erwin, Houston C. Chandler, John G. Palis, Thomas A. Gorman, and Carola A. Haas
Southeastern Naturalist, Volume 15, Issue 3 (2016): 431–447
Full-text pdf (Accessible only to subscribers.To subscribe click here.)
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K.J. Erwin, H.C. Chandler, J.G. Palis, T.A. Gorman, and C.A. Haas
22001166 SOUTHEASTERN NATURALIST 1V5o(3l.) :1453,1 N–4o4. 73
Herpetofaunal Communities in Ephemeral Wetlands
Embedded within Longleaf Pine Flatwoods of the Gulf
Coastal Plain
Kenneth J. Erwin1, Houston C. Chandler1,2,*, John G. Palis3, Thomas A. Gorman1,4,
and Carola A. Haas1
Abstract - Ephemeral wetlands surrounded by Pinus palustris (Longleaf Pine) flatwoods
support diverse herpetofaunal communities and provide important breeding habitat for
many species. We sampled herpetofauna in 3 pine flatwoods wetlands on Eglin Air Force
Base, Okaloosa County, FL, over 2 time periods (1 wetland [1] from 1993 to1995 and 2
wetlands [2 and 3] from 2010 to 2015) using drift fences that completely encircled each
wetland. We documented 37, 46, and 43 species of amphibians and reptiles at wetlands 1,
2, and 3, respectively. Herpetofaunal communities were remarkably similar across all 3
wetlands (Sorenson Index values > 0.97) despite sampling that occurred 15–20 years apart
on wetlands located approximately 10 km apart. Ambystoma bishopi (Reticulated Flatwoods
Salamander), Pseudacris ornata (Ornate Chorus Frog), and Eurycea quadridigitata
(Dwarf Salamander), all species of conservation concern, were captured at all 3 wetlands,
indicating that these wetlands provide habitat for specialist species. Overall, habitat conservation
and management has succeeded in maintaining suitable habitat for herpetofauna
in recently surveyed wetlands, despite continued range-wide threats from changes to historic
fire regimes and climate change.
Introduction
Ephemeral wetlands often support diverse amphibian and reptile communities
(Dodd and Cade 1998, Gibbons et al. 2006, Means et al. 2004). Herpetofaunal diversity,
especially breeding amphibians, can be an indicator of overall ecosystem
quality (Welsh and Droege 2001, Welsh and Ollivier 1998). Many amphibians
migrate from surrounding uplands to ephemeral wetlands to reproduce because
ephemeral wetlands provide high-quality breeding habitat (Capps et al. 2015, Duellman
and Trueb 1986, Palis 1997). A regular drying phase excludes predatory
fishes from ephemeral wetlands, which reduces the predation pressure on larval amphibians
when compared to permanent water bodies (Leibowitz 2003, Skelly 1997).
Furthermore, the breeding phenology of multiple amphibian species often overlaps,
creating a complex aquatic community and an abundant source of prey for other
species (e.g., Lawler and Morin 1993). Predators of amphibians include many species
of reptiles, which often opportunistically forage in ephemeral wetlands during
certain times of the year (Eskew et al. 2009, Preston and Johnson 2012, Russell et
1Department of Fish and Wildlife Conservation, Virginia Tech, Blacksburg, VA 24061. 2 The
Orianne Society, Tiger, GA 30576. 3Palis Environmental Consulting, PO Box 387, Jonesboro,
IL 62952. 4 Aquatic Resources Division, Washington State Department of Natural
Resources, Chehalis, WA 98532. *Corresponding author - houstonc@vt.edu.
Manuscript Editor: Karen Francl
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al. 2002a). High amphibian reproductive output combined with an influx of outside
predators can link ephemeral wetlands to surrounding uplands (e.g., Regester et al.
2006, Schriever et al. 2014, Whiles et al. 2006).
Globally, amphibian and reptile populations have experienced severe declines
in recent years because of a variety of factors including habitat alteration, climate
change, pollution, and disease (Carey and Alexander 2003, Gibbons et al. 2000,
Stuart et al. 2004). Degraded wetland quality can have negative impacts on amphibian
(Gorman et al. 2013, McMenamin et al. 2008) and reptile (Aresco 2005,
DeCatanzaro and Chow-Fraser 2010) communities. The loss of natural-disturbance
regimes can alter wetland habitat (even on managed lands), creating unsuitable
habitat patches for some amphibian species (Gorman et al. 2013, Martin and
Kirkman 2009). Wetland hydroperiod also plays an important role in determining
amphibian breeding success (Semlitsch et al. 1996), and persistent drought can
cause reproductive failure for entire populations (Palis et al. 2006, Westervelt et
al. 2013). Thus, long-term monitoring of herpetofaunal communities in wetland
habitats is important for identifying population trends over time and for describing
the communities that utilize these wetlands.
The southeastern United States is characterized by abundant wetland resources
and a high diversity of wetland habitats (Sutter and Kral 1994). Included in this
diverse wetland assemblage are isolated ephemeral wetlands embedded within
Pinus palustris Mill. (Longleaf Pine) flatwoods, which are found in low-lying areas
of the Coastal Plain (Means 1996). Longleaf Pine forests have been reduced
to approximately 3% of their original extent because of widespread anthropogenic
disturbance that occurred after European settlement (Means 1996). The decline
of Longleaf Pine forests has led to the loss and degradation of wetlands that were
historically embedded within these systems (Hefner and Brown 1984). Today, remaining
pine flatwoods wetlands are often degraded to varying degrees as a result
of continued anthropogenic disturbance, a loss of historic fire regimes in these fireadapted
ecosystems, and climate change (Brooks 2009, Gorman et al. 2013).
We report on herpetofaunal communities surveyed in 3 pine flatwoods wetlands
in western Florida. We compare herpetofaunal communities among these 3 wetlands
and through time for the 2 wetlands sampled between 2010 and 2015. We
also examine the relative abundance of several species, and we focus specifically
on 3 species of conservation concern: Ambystoma bishopi (Reticulated Flatwoods
Salamander), Pseudacris ornata (Ornate Chorus Frog), and Eurycea quadridigitata
(Dwarf Salamander). Reticulated Flatwoods Salamanders breed exclusively in
pine flatwoods wetlands and were listed as federally endangered in 2009 (USFWS
2009). The Ornate Chorus Frog is classified as a species of greatest conservation
need in Florida because of recent population declines (Florida Fish and Wildlife
Conservation Commission 2005). The Dwarf Salamander is also designated as a
species of greatest conservation need in Florida (Florida Fish and Wildlife Conservation
Commission 2005) and is believed to represent a species complex (Harrison
and Guttman 2003).
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2016 Vol. 15, No. 3
Figure 1. Three pine flatwoods wetlands on Eglin Air Force Base, Okaloosa County, FL, that
were each completely encircled by a drift fence shown on a digital elevation model (darker
areas represent higher elevations). The boundary between shaded and unshaded areas is the
coastline (shaded area is land and the white area is water). Wetland 1 was monitored from
1993 to 1995 (2 seasons) and was located in East Bay Flatwoods. Wetlands 2 and 3 were
sampled from 2010 to 2015 (5 seasons) and were located in Oglesby’s Flatwoods (~10 km
from wetland 1).
Field-site Description
We sampled 3 pine flatwoods wetlands located on Eglin Air Force Base (AFB)
in Okaloosa County, FL. One wetland (1) was sampled for 2 seasons (1993–1995),
and the other 2 wetlands (2 and 3) were sampled for 5 seasons (2010–2015). Wetland
1 had a wetted area of approximately 0.8 ha, and wetlands 2 and 3 had a wetted
area of approximately 0.4 ha each. Eglin AFB, a large military installation, contains
over 187,000 ha of various habitat types, including over 145,000 ha of actively
managed Longleaf Pine forests. Wetland 1 was located in East Bay flatwoods, and
wetlands 2 and 3 in Oglesby’s Flatwoods, approximately 10 km from wetland 1
(Fig. 1). These wetlands are typically filled by winter rains (November–February)
and then experience a dry period during late spring or early summer (Chandler et al.
2016). They are characterized by open canopies composed mostly of Pinus elliottii
Engelm. (Slash Pine), Longleaf Pine, and Taxodium ascendens Brongn. (Pond Cypress).
Wetland midstories are dominated by shrub species including Ilex myrtifolia
Walter (Myrtle Dahoon) and Hypericum chapmanii W.P. Adams (Apalachicola St.
Johnswort). Wetland basins are dominated by thick herbaceous vegetation including
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Table 1. Dates of operation for 3 drift fences encircling pine flatwoods wetlands on Eglin Air Force
Base, Okaloosa County, FL. The number of trap nights (i.e., one trap set for one night) for each season
is indicated in parentheses.
Season Wetland 1 Wetland 2 Wetland 3
1993–1994 10/9–3/31 (9060) - -
1994–1995 10/27–5/19 (9462) - -
2010–2011 - 11/2–2/27 (2771) 12/12–2/27 (1960)
2011–2012 - 10/31–3/23 (8928) 10/31–3/23 (8064)
2012–2013 - 10/29–3/12 (7498) 10/29–3/12 (6762)
2013–2014 - 10/6–5/31 (13,212) 10/6–5/31 (11,928)
2014–2015 - 9/13–3/29 (10,553) 9/13–3/29 (9536)
Aristida stricta Michx. (Pineland Threeawn), Eriocaulon compressum Lam. (Flattened
Pipewort), and Andropogon spp. (bluestems) (Chandler 2015; Gorman et al.
2013, 2014).
Methods
We constructed drift fences that completely encircled each wetland. Palis
(1997) described the drift-fence installation methods used for the surveys conducted
at wetland 1 from 1993 to 1995, and we used similar methods for the
surveys at wetlands 2 and 3 from October 2010 to May 2015. At wetlands 2 and 3,
we installed drift fences using 60-cm high rolls of galvanized steel flashing, and
we buried approximately 15–20 cm of the flashing to reduce the chances of incidental
trespass under the fences. Each drift fence had a different number of funnel
traps that, depending on the size of the wetland and the length of the fence, were
spaced approximately 10 m apart and placed in pairs along both sides of the fence
(i.e., for each pair, one trap was placed on the inside of the fence and a second on
the outside). Wetlands 1, 2, and 3 had 31, 31, and 28 pairs of funnel traps, respectively.
We constructed all of the funnel traps using aluminum window screening.
Funnel traps were approximately 85 cm x 20 cm with a funnel on each end that
had a 5-cm opening. We used two wooden stakes to hold each trap in place along
the drift fence so that traps were pressed firmly against the ground and the fence.
Finally, we placed a wet sponge in each trap (to reduce the chances of desiccation
of amphibians) and covered all traps with a 61 cm x 61 cm shade board to further
reduce the chance of mortality.
The dates that drift fences were run varied across wetlands and through time,
but, in general (except for 2010), we set all traps prior to the first heavy rainfall
event in October (Table 1). We ran drift fences until mid- to late spring before
closing all traps. In the figure and tables presented herein, a “drift fence season”
is defined as the start of data collection in the fall of one year and ending with late
spring sampling in the following year, with each season referenced by the year in
which the sampling period ended (e.g., 1994 = data collected fall 1993 to spring
1994, a complete drift-fencing season). Where possible in the text, we indicate timing
of events by prefacing the year with F (fall) or S (spring).
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At wetlands 2 and 3, we checked traps every evening beginning just after sunset.
To minimize potential trap mortality from Solenopsis invicta Buren (Red Fire
Ant) predation, we monitored soil temperatures at 2 cm depth to determine the
likelihood of foraging activity (Porter and Tschinkel 1987). On dry nights (low
likelihood of salamander movement) with soil temperatures above 15 °C (high
likelihood of fire ant foraging), we checked traps continuously until approximately
midnight and once after dawn. On dry nights when soil temperatures were <15 °C,
we performed an initial check in the evening and once after dawn. On rainy nights
when soil temperatures were >15 °C, we checked drift fences every 2 hours for the
entire night until dawn. At all 3 wetlands, we identified all captured amphibians and
reptiles to species and recorded each individual’s stage and sex class (adult male,
adult female, or juvenile) and whether females were gravid or not, when possible.
We also were able to age some Reticulated Flatwoods Salamanders as yearlings
based on the retention of a lateral line (following Palis 1997). All animals were
released on the opposite side of the fence from where they were captured.
We used the abundance-based Sorenson index (Ss) to calculate similarity indices
among the 3 wetlands (Krebs 1998). The Sorenson index uses species lists and the
relative abundance of each species to calculate a similarity value for 2 sites. Values
range from 0 to 1 with values closer to 1 indicating more-similar communities. We
calculated relative abundances separately for each wetland and reported those metrics
as the percentage of the total captures (pooled across years) of a given species.
Results
Herpetofaunal diversity was high across all 3 wetlands and in a majority of
sampling seasons (though fewer trap nights [i.e., 1 trap set for 1 night] were conducted
from F2010 to S2011; Table 1, Fig. 2). From F1993 to S1995, we captured
37 species of amphibians and reptiles in wetland 1, and from F2010 to S2015, we
captured 46 and 43 species in wetlands 2 and 3, respectively (Appendix 1). Species
richness did vary across years from F2010 to S2015, and F2013–S2014 had
the highest species richness of any season, which coincided with above average
precipitation during that year. Furthermore, all 3 wetlands had similar communities
when captures were pooled for each site (Ss for wetlands 1 and 2 = 0.98, Ss for
wetlands 1 and 3 = 0.98, and Ss for wetlands 2 and 3 = 0.99).
Even though community composition was similar among all sites, there were
differences in the relative abundance of the most common species in each wetland
(Fig. 3). In wetland 1, Dwarf Salamanders were by far the most abundant species
(56.7% of total captures), and the next most abundant species was Reticulated
Flatwoods Salamanders, which accounted for only 7.1% of total captures. Acris
gryllus (Southern Cricket Frog) and Lithobates sphenocephalus (Southern Leopard
Frog) were the 2 most abundant species in wetlands 2 and 3 (Fig. 3). Dwarf
Salamanders accounted for only 3.8% and 6.1% of total captures in wetlands 2 and
3, respectively. Kinosternon subrubrum (Eastern Mud Turtle) was the only reptile
species included in the 8 most abundant species for any wetland (only in wetland
1). Other commonly encountered reptile species included Agkistrodon piscivorus
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(Cottonmouth), Regina rigida (Glossy Crayfish Snake), and Scincella lateralis
(Ground Skink). From F2010 to S2015, Cottonmouths were most abundant during
F2013–S2014 (76% of all Cottonmouths captured). Sixty-two percent of total
Cottonmouth captures from F2010 to S2015 occurred from March to May 2014.
F2013–S2014 had highest rainfall and therefore a large emergence of metamorphic
amphibians, and was the only successful Reticulated Flatwoods Salamander breeding
year from F2010 to S2015.
We captured Reticulated Flatwoods Salamanders, Ornate Chorus Frogs, and
Dwarf Salamanders in every sampling season at all 3 wetlands. From F2010 to
S2015, Reticulated Flatwoods Salamanders migrated to wetlands in October, November,
or December, which typically coincided with the first rains of the season
(Fig. 4). In February 2013, we observed a large movement out of wetland 2 following
a large rain event that filled the previously dry site. In spring 2014, large
numbers of Reticulated Flatwoods Salamander metamorphs were captured leaving
the wetlands during April and May (Fig. 4). We have documented yearlings in 4
Figure 2. Herpetofaunal species richness in 3 pine flatwoods wetlands on Eglin Air Force
Base, Okaloosa County, FL, sampled using a drift fence that completely encircled each
wetland. Wetland 1 was sampled for 2 seasons (1993–1995), and wetlands 2 and 3 were
sampled for 5 seasons (2010–2015). Sampling seasons usually started in October, and fences
were usually closed in either March or May, depending on the conditions in the wetlands.
Years are referenced by the January through close portion of the sampling season. See Table
1 for the dates of sampling and number of trap nights in each year. The low species richness
in 2011 is likely an artifact of a short sampling season in that year.
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Figure 3. The relative abundance (percent of total captures) of the 8 most abundant herpetofauna
captured in drift fences encircling 3 pine flatwoods wetlands (~90% of total captures
in all 3 wetlands). Wetland 1 was sampled October–March (1993–1994) and October–May
(1994–1995), and wetlands 2 and 3 were sampled for 5 seasons (2010–2015) over approximately
the same months. All 3 wetlands were located on Eglin Air Force Base, Okaloosa
County, FL. Species codes represent the first three letters of the genus followed by the
first three letters of the species name (ACRGRY = Acris gryllus; AMBBIS = Ambystoma
bishopi; ANATER = Anaxyrus terrestris; EURQUA = Eurycea quadridigitata; GASCAR
= Gastrophryne carolinensis; KINSUB = Kinosternon subrubrum; LITSPH = Lithobates
sphenocephalus; NOTVIR = Notophthalmus viridescens; PSENIG = Pseudacris nigrita;
PSEORN = Pseudacris ornata).
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Figure 4. Total weekly captures from 2011 to 2015 for Ambystoma bishopi (Reticulated Flatwoods Salamander), Eurycea quadridigitata
(Dwarf Salamander), and Pseudacris ornata (Ornate Chorus Frog) at drift fences encircling 2 pine flatwoods wetlands on Eglin Air Force
Base, Okaloosa County, FL. Two weeks for wetland 2 had greater than 75 P. ornata captures, and the actual number of captures is indicated
in parentheses on the graph. Captures for E. quadradigitata were low until the 2014–2015 season. Weekly precipitation totals are plotted
over the same time period. Grey bars separate sampling seasons.
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2016 Vol. 15, No. 3
seasons, suggesting that the prior year was a successful breeding season (i.e., detection
of yearlings occurred in F1993, F1994, F2010, and F2014). Ornate Chorus
Frogs displayed similar movement patterns to Reticulated Flatwoods Salamanders,
with large movements generally coinciding with the first heavy autumn rains of the
season. Finally, Dwarf Salamander captures varied over time and among wetlands.
In wetlands 2 and 3, only small numbers of Dwarf Salamanders were captured
from F2010 to S2014 (F2010–S2011: 58, F2011–S2012: 28, F2012–S2013: 26,
F2013–S2014: 43), but there were 363 captures in F2014–S2015 for the 2 wetlands
combined (Fig. 4). In wetland 1, there were 2055 Dwarf Salamander captures in
F1993–S1994 and 1341 captures in F1994–S1995.
Discussion
We documented diverse herpetofaunal communities in 3 pine flatwoods wetlands
over multiple sampling seasons. Other drift-fence surveys have documented
similar herpetofaunal diversity in Coastal Plain wetlands of the southeastern US
(e.g., Dodd [1992]: 42 species in a northeast Florida wetland, Russell et al. [2002b]:
56 species combined in 5 wetlands in South Carolina). Furthermore, drift-fence
surveys of steephead ravines on and around Eglin AFB documented 44 species of
reptiles and amphibians (Enge 2005), and drift-fence surveys in flatwoods and hammocks
of western Florida captured 53 species of reptiles and amphibians from 2002
to 2005 (Dodd et al. 2007). We observed remarkably similar herpetofaunal communities
between all wetlands (all Ss > 0.97) despite the 15–20-year gap in sampling
events and the different locations of wetlands included in this study. This result
indicates that these wetlands supported a general pool of species that accounted
for the vast majority of captures in this study (8 species accounted for >85% of
all captures). Species that were not captured at all sites were only captured a few
times and likely do not depend on these wetlands during the times of year in which
we sampled, if at all. For example, Apalone ferox (Florida Softshell), captured a
total of just 9 times and at only 1 wetland, usually prefer more permanent water
bodies, and Plethodon grobmani (Southeastern Slimy Salamander), captured a total
of 3 times at 2 wetlands, is an upland species that does not migrate to ephemeral
wetlands to breed (Conant and Collins 1998). High community similarity among
wetlands combined with high species richness during a majority of years demonstrates
that pine flatwoods wetlands reliably support highly diverse herpetofaunal
communities that can contribute to regional diversity (Means et al. 2004, Russell et
al. 2002b).
High herpetofaunal diversity at sites surveyed in recent years indicates that
habitat management on Eglin AFB has been successful for maintaining wetland
resources, despite changes to historic fire regimes. Eglin AFB has one of the most
active prescribed burning programs in the country (Eglin Air Force Base 2010),
which creates and maintains high-quality upland habitat. However, there are challenges
associated with prescribed burning in wetlands (e.g., prescribed fires are
frequently set during the dormant season when wetlands are inundated; Bishop and
Haas 2005). The inability of dormant-season fires to burn through wetland basins
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has resulted in some wetlands becoming so degraded that a more-active management
strategy is required to restore high-quality vegetation communities (e.g.,
Gorman et al. 2009, 2013; Martin and Kirkman 2009). Recent active management
may have contributed to the high species richness seen in wetlands 2 and 3. Despite
the degradation that can occur with dormant-season fires, it is not uncommon for
wetlands to retain some characteristic vegetation structure (i.e., some edge habitat
usually burns even during dormant-season fires), and it is unlikely that wetlands
2 and 3 would support the diverse herpetofauna that we documented if not for the
habitat management that has occurred on Eglin AFB.
Even though the overall herpetofaunal assemblages were similar among wetlands,
we did observe differences in the relative abundances of certain species.
Dwarf Salamanders were an order of magnitude more abundant in wetland 1 compared
to wetlands 2 and 3. We cannot determine whether the large difference in
abundance reflects differences in locations surveyed or changes over time or both.
Other surveys (larval dipnetting) from F2010 to S2015 at both locations indicate
that East Bay Flatwoods (wetland 1) does support a higher abundance of Dwarf
Salamander when compared to Oglesby’s Flatwoods (wetlands 2 and 3; T. Gorman
and C. Haas, Virginia Tech, Blacksburg, unpubl. data). The substantial increase in
Dwarf Salamander captures in wetlands 2 and 3 after a single year with above average
precipitation does suggest that more successful reproduction occurred during
F2013–S2014 than F2010–S2013. We observed a similar trend in the Reticulated
Flatwoods Salamander populations at wetlands 2 and 3. The number of adult salamanders
declined from F2010 to S2015 to a low of just 23 and 14 (wetlands 2 and
3, respectively) during F2014–S2015, likely because of multiple reproductive failures
during drought years (Chandler et al. 2016, Palis et al. 2006, Westervelt et al.
2013). We did observe one successful breeding season (metamorphs leaving wetlands
2 and 3 in S2014), which increased the total number of Reticulated Flatwoods
Salamander captures to 116 and 89 (wetlands 2 and 3, respectively) F2014–S2015
(adults and yearlings combined). However, it is likely that populations in these 2
wetlands may be almost completely composed of a single cohort in subsequent
years. These examples highlight the challenges for amphibians breeding in ephemeral
wetlands (Dodd 1992, Gibbons et al. 2006, Semlitsch et al. 1996), especially
as climate change continues to affect wetland hydrology (Chandler et al. 2016).
An increased frequency of drought and shortened or more unpredictable hydroperiods
are likely to be primary stressors for these wetland communities in the future,
especially in landscapes where anthropogenic degradation is minimal (Chandler et
al. 2016, Walls et al. 2013, Westervelt et al. 2013). Palis et al. (2006) documented
how severe drought can lead to complete reproductive failure (no metamorphosis)
in flatwoods salamander populations, and the effects of drought are likely similar for
other habitat specialists that breed in ephemeral wetlands. During our surveys, we
sampled during multiple years that were characterized by drought and short hydroperiods
(e.g., F2012–S2013 and F2014–S2015). Drought years did have a species
richness similar to other non-drought years, and wetlands did fill for at least a short
time during all sampling seasons (although years with no standing water in these
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wetlands have been documented; Gorman et al. 2009). Observing only 1 successful
breeding event by Reticulated Flatwoods Salamanders during the period F2010–
S2015 and the increase in Dwarf Salamander captures after a year with above average
rainfall indicate that, for drift-fence studies to be effective in these systems, they
need to span enough years to capture the temporal variation of ephemeral-wetland
breeding species (Palis and Aresco 2007, Semlitsch et al. 1996).
Effectively monitoring herpetofaunal communities in pine flatwoods wetlands
is important for several reasons. In ephemeral wetlands, larval amphibians play an
important role in aquatic food webs, serving as prey sources for predatory invertebrates
and vertebrates and directly competing with both herbivorous (Beard et
al. 2003, Morin et al. 1988) and predaceous invertebrates (Caldwell et al. 1980).
Larval amphibians can also affect nutrient cycling within a wetland and alter
decomposition rates (Capps et al. 2015, Hocking and Babbitt 2014, Whiles et al.
2006). Furthermore, amphibian breeding events serve as one of the primary links
between wetland energy sources and surrounding upland forests (Schriever et al.
2014, Whiles et al. 2006), and these breeding events provide resources for numerous
other species, including many reptiles (Wilbur 1997). Given the extensive loss
of wetland resources across the southeastern United States (Hefner and Brown
1984), the importance of wetlands within pine flatwoods to herpetofaunal diversity,
and the current threats to these ecosystems, supporting high-quality vegetation
structure through regular fires should be a management priority for the remaining
pine flatwoods wetlands (Bishop and Haas 2005; Chandler et al. 2015; Gorman et
al. 2009, 2013). Finally, detailed research (e.g., Chandler et al. 2016, McLaughlin
and Cohen 2014) is needed in these and other ephemeral wetlands to better understand
the drivers and threats to wetland hydrologic processes.
Acknowledgments
We thank the many people who have assisted with this work, especially C. Abeles, D.
Atencio, A. Austermann, J. Bell, A. Daniels, M. Dziadzio, L. Gamble, K. Gault, P. Gault, S.
Goodman, B. Hagedorn, J. Hefner, A. Hillman, J. Jensen, J. Johnson, K. Jones, L. LaClaire,
C. Mahmood, T. Mammone, A. Minor, H. Mitchell, B. Moore, J. Newman, C. Nordman,
E. Nordman, B. O’Hanlon, E. Ohr, J. Parker, A. Perez-Umphrey, C. Petrick, J. Preston, A.
Saenger, S. Powell, B. Rincon, S. Ritchie, J. Da Silva Neto, and T. Williams. We thank
Karen Powers, Susan Walls, and 1 anonymous reviewer for their helpful comments on an
earlier draft of this manuscript. Also, we thank the Natural Resources Branch of Eglin Air
Force Base (Jackson Guard), Department of Defense Legacy Resource Management Program,
Hurlburt Field, US Fish and Wildlife Service, Aquatic Habitat Restoration/Enhancement
Program of Florida Fish and Wildlife Conservation Commission, and the Department
of Fish and Wildlife Conservation at Virginia Tech for financial and logistical support of
this project. Sampling protocols were approved by the Virginia Tech Institutional Animal
Care and Use Committee (IACUC), Protocol no. 13-129-FIW.
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Appendix 1. Herpetofauna captured in drift fences that encircled 3 pine flatwoods ponds on Eglin Air Force Base, Okaloosa County, FL. Wetland 1 was
sampled for 2 seasons (1993–1995), and wetlands 2 and 3 were sampled for 5 seasons (2010–2015). Drift fences were usually opened in October and run
through March or May of the following year (the spring portion of the year is used to reference seasons).
Wetland 1 Wetland 2 Wetland 3
Species 1994 1995 2011 2012 2013 2014 2015 2011 2012 2013 2014 2015
Frogs and Toads
Acris gryllus (LeConte) (Southern Cricket Frog) 69 153 53 24 369 706 136 13 20 274 783 312
Anaxyrus quercicus (Holbrook) (Oak Toad) 14 40 2 7 20 3 3 51 1
Anaxyrus terrestris (Bonnaterre) (Southern Toad) 65 17 10 167 15 75 12 3 55 18 226 34
Eleutherodactylus planirostris (Cope) (Greenhouse Frog) 1 1 11 25 8 1 18 5
Gastrophryne carolinensis (Holbrook) (Eastern Narrow-mouthed Toad) 47 94 11 55 138 257 104 32 67 346 61
Hyla cinerea (Schneider) (Green Treefrog) 2 1
Hyla femoralis Bosc in Daudin (Pine Woods Treefrog) 3 43 1 2 3 15 11 8 26 8
Hyla gratiosa (LeConte) (Barking Treefrog) 1
Hyla squirella Bosc in Daudin (Squirrel Treefrog) 4 1 2
Lithobates capito (LeConte) (Gopher Frog) 2 3
Lithobates clamitans (Latreille in Sonnini de Manoncourt and Latreille) 2 12 20
(Green Frog)
Lithobates grylio (Stejneger) (Pig Frog) 4 8 4 15 6
Lithobates sphenocephalus (Cope) (Southern Leopard Frog) 91 111 9 32 106 713 69 10 17 52 417 39
Pseudacris nigrita (LeConte) (Southern Chorus Frog) 149 137 2 3 3 9 3 1 3
Pseudacris ornata (Holbrook) (Ornate Chorus Frog) 173 75 79 37 241 282 145 24 23 32 169 41
Scaphiopus holbrookii (Harlan) (Eastern Spadefoot) 12 6 2 2 6 1 1 8
Salamanders
Ambystoma bishopi Goin (Reticulated Flatwoods Salamander) 349 93 188 145 92 235 217 29 86 36 114 151
Amphiuma means (Garden in Smith) (Two-toed Amphiuma) 1 1 1
Eurycea quadridigitata (Holbrook) (Dwarf Salamander) 2055 1341 42 5 17 34 138 16 23 9 9 225
Notophthalmus viridescens (Rafinesque) (Eastern Newt) 101 66 35 82 58 101 271 18 20 36 62 130
Plethodon grobmani Allen and Neill (Southeastern Slimy Salamander) 2 1
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Wetland 1 Wetland 2 Wetland 3
Species 1994 1995 2011 2012 2013 2014 2015 2011 2012 2013 2014 2015
Lizards
Anolis carolinensis Voigt (Green Anole) 12 6 2 2 2 8 2
Cnemidophorus sexlineatus (L.) (Six-lined Racerunner) 6 1 2
Ophisaurus attenuatus Cope (Slender Glass Lizard) 3
Ophisaurus ventralis (L.) (Eastern Glass Lizard) 2 3 3 9 5 5 16 5
Plestiodon anthracinus Baird (Coal Skink) 2 1
Plestiodon laticeps (Schneider) (Broad-headed Skink) 1 2 3 1 3
Sceloporus undulatus Bosc and Daudin in Sonnini and Latreille 1 4
(Eastern Fence Lizard)
Scincella lateralis (Say) (Ground Skink) 70 19 1 4 2 6 1 12 1 10 1
Snakes
Agkistrodon piscivorus (Lacépède) (Eastern Cottonmouth) 5 10 40 7 40 3
Cemophora coccinea (Blumenbach) (Scarlet Snake) 1 1 2 2
Coluber constrictor L. (Black Racer) 32 15 1 2 1 5 1 1 2 3
Diadophis punctatus (L.) (Ring-necked Snake) 8 1 4 7 1 1 2
Farancia abacura (Holbrook) (Mud Snake) 7 2 1 1
Heterodon platirhinos Latreille in Sonnini and Latreille (Eastern 3 3 2 1
Hog-nosed Snake)
Lampropeltis elapsoides (Holbrook) (Scarlet Kingsnake) 1 2
Lampropeltis getula (L.) (Eastern Kingsnake) 4
Nerodia fasciata (L.) (Banded Water Snake) 13 21 3 8 44 2 1 4 5 22 6
Opheodrys aestivus (L.) (Rough Green Snake) 1 1 1 1 2
Pantherophis guttatus (L.) (Corn Snake) 1 2 1 3 9 7 4 2 2 5
Regina rigida (Say) (Glossy Crayfish Snake) 16 9 19 47 2 2 27 46
Sistrurus miliarius (L.) (Pygmy Rattlesnake) 1
Storeria occipitomaculata (Storer) (Red-bellied Snake) 1 2 1
Tantilla coronate Baird and Girard (Southeastern Crowned Snake) 1 1 1 3 4
Thamnophis sauritus (L.) (Eastern Ribbon Snake) 27 44 4 1 5 36 20 2 4 19 14
Thamnophis sirtalis (L.) (Common Garter Snake) 19 10 4 3 2 6 9 2 4 10
Virginia valeriae Baird and Girard (Smooth Earth Snake) 1 1 2
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Wetland 1 Wetland 2 Wet
Species 1994 1995 2011 2012 2013 2014 2015 2011 2012 2013 2014 2015
Turtles
Apalone ferox (Schneider) (Florida Softshell) 2 7
Deirochelys reticularia (Latreille in Sonnini and Latreille) (Chicken 5 7 5
Turtle)
Kinosternon subrubrum (Bonnaterre) (Eastern Mud Turtle) 94 243 3 5 12 10 12 1 8 7 7 6
Pseudemys floridana (LeConte) (Coastal Plain Cooter) 13
Terrapene carolina (L.) (Eastern Box Turtle) 6 9 2 1 1 1
Trachemys scripta (Thunberg in Schoepff) (Pond Slider) 5 2 14